In vitro antioxidant activities of different extract (pet. ether, chloroform, ethylacetate, alcohol residue) of aerial parts of Caesalpinia mimosoides Lamk was investigated. The free radical scavenging activity to evaluate by Iron chelating method, Nitric oxide method. In iron chelating assay the IC50 value of the standard ascorbic acid was found to be 49.70μg/ml and it was comparable with the IC50 value of EAE (IC50 58.32μg/ml). The nitric oxide scavenging ability of ethyl acetate extract IC50 value was found to be 61.77μg/ml which is comparable with the ascorbic acid (32.37μg/ml). Ethyl acetate extract exhibited good antioxidant activity when compared to other extracts. The results of the study showed that aerial parts of caesalpina mimosoides Lamk. Have strong radical scavenging and reducing capacities.
In vitro antioxidant activities of different extract (pet. ether, chloroform, ethylacetate, alcohol residue) of aerial parts of Caesalpinia mimosoides Lamk was investigated. The free radical scavenging activity to evaluate by Iron chelating method, Nitric oxide method. In iron chelating assay the IC50 value of the standard ascorbic acid was found to be 49.70μg/ml and it was comparable with the IC50 value of EAE (IC50 58.32μg/ml). The nitric oxide scavenging ability of ethyl acetate extract IC50 value was found to be 61.77μg/ml which is comparable with the ascorbic acid (32.37μg/ml). Ethyl acetate extract exhibited good antioxidant activity when compared to other extracts. The results of the study showed that aerial parts of caesalpina mimosoides Lamk. Have strong radical scavenging and reducing capacities.
The present study is to investigate the nephrolithiasis produced by topiramate in male albino rats. For this study the male albino rats were divided into three groups containing six animals in each and received distilled water, Topramate 4.5mg/kg, Topiramate 18mg/kg orally for 28 days. On 14th and 28th day, the following parameters like serum urea, BUN, creatinine, urine calcium, phosphorus, creatinine, uric acid, antioxidant enzymes MDA, GSH, SOD, catalase and renal histopathology were monitored after the treatment period. Statistical analysis was done by one way ANOVA with Dunnet multiple comparison test. There was a significant change in urine volume, kidney weight and elevated serum urea, creatinine, calcium and phosphorus as compared to control rats. Lipid peroxidation products MDA were significantly increased. Antioxidant enzymes SOD, GSH and catalase levels were significantly lowered than the control group. Histopathological study of kidney also supported the above results. These observations enable us to conclude that topiramate produces nephrolithiasis as dose dependent manner.
