We aimed to develop the yield and biological properties series of (E)-N-((1H-indol-3-yl) methylene)-benzamine derivatives were synthesized by assisted by Bronsted acid taking polar protic solvent. All the newly synthesized compounds were analyzed by spectra data viz; IR, 1H NMR and LCMS. All newly compounds were tested against three gram positive and two gram-negative bacterial strains and one in fungal strain. All newly compounds showed good activity against gram positive strains than against gram negative strains. The newly compounds were found more active against S.aureus and B.subtilis

Urinary tract infection is one of the most common disease in both male and female of all age groups. Both gram positive and gram negative bacterial pathogens are responsible for UTI. In this study, 25 Urethral samples were collected. Among that, 34 pathogens were isolated. The isolates were identified and confirmed by biochemical characterisation tests and culturing method. Totally seven types of bacterial pathogens were isolated namely E.coli, P.aeuruginosa, K.pneumonia, P.vulgaris, P.mirabilis, S.aureus and S.saprophyticus. The most common pathogens affected by the individuals were Klebsiella and E.coli. These pathogens were treated with different antibiotics and the findings were recorded.

Elicitation is an efficient tool to enhance/induce both chemical, and physiological changes which may subsequently influence the biological activity. This may stimulate both defense and stress-induced responses in many important medicinal plants and foods. Elicitors from different origins could be used either alone or with hydroponic sprays during growth or right before harvest. A better knowledge on the effect of certain compounds on biosynthetic pathways in responding to specific treatments with elicitors, would be a very useful way to augment the production of secondary metabolites or produce new metabolites. This will help in production a high quality, healthy, and useful medicinal plants and foods. Moringa oleifera (MO) leaves extract contains a several active constituents; alkaloids, carotenoids, glucosinolates, polyphenols, tannins and saponins and considered as a good biotic elicitor. It was used in this study to enhance both phenolic and antioxidant contents in germinated alfalfa sprout. In order to determine the ideal concentration for maximum elicitor response, various dilutions were tested (0.5, 0.25, 0.125, 0.0625, 0.03125 and 0.015625g/L). Germination of alfalfa seeds in continuous light and soaking seeds in 0.0625, 0.03125g/L MO extract before germination significantly increases the levels of total phenolics and their antioxidant activity. The maximum amount of flavonoids were exudate after 8 hours of germination. The optimal concentration to elicit maximum phenolic levels was further used to study the biological activities.