The solubility of the drug is essential for the formulation and development of the drug. One of the most important pre-formulation parameters is the solubility of drug. The drug molecules must be adequately solubilized for parenteral preparations. Solubility and permeability are also essential for the bioavailability of solid formulations like tablets and capsules. The aim of the study was to use a UV-visible spectrophotometer to examine the solubility of the drug in various pH environments. Buffers with a pH range of 1.2 to 7.4 and also distilled water were used for studying the solubility of drug. The results of this study confirmed that the solubility of Ranolazine is pH-dependent.

This study focused on the acquisition, authentication and comprehensive evaluation of Crossandra infundibuliformis (L.), a medicinal plant collected from the Jamia Salafiya Pharmacy College Campus in Kerala, South India. Thorough authentication by experts from the Institute of Forest Genetics and Tree Breeding, Coimbatore, ensured the reliability of the plant material, which was deposited in the Fischer Herbarium under accession number 25233.An ethanolic Soxhlet extraction method was employed to extract bioactive compounds from finely ground and dried leaves of Crossandra infundibuliformis (L.). This technique allows for the efficient extraction of phytochemicals and secondary metabolites. The resulting extract exhibited a semi-solid consistency with a distinctive brown colour, indicating the presence of specific compounds or pigments. In vitro antioxidant assays using the DPPH radical scavenging method revealed the significant antioxidant activity of the ethanolic extract. Ascorbic acid, a standard antioxidant, exhibited an IC₅₀ of 1.562µg/mL, whereas the extract demonstrated a slightly higher IC₅₀ of 12.5µg/mL, emphasising its substantial antioxidant potential. The antibacterial activity of the ethanolic extract was evaluated using the well-diffusion method, which showed notable inhibition zones, particularly against Staphylococcus aureus (17mm). The efficacy of this extract is comparable to that of tetracycline. Electron microscopic examination confirmed bacterial cell lysis and shrinkage, supporting the antibacterial activity of the ethanolic extract. The inhibitory concentration was determined using a minimal inhibitory concentration (MIC) assay, further highlighting the antimicrobial potential of the extract. Additionally, the ethanolic extract exhibited in vitro inflammatory activity by promoting the migration rate of fibroblasts in a scratch assay, suggesting its potential wound healing properties. In conclusion, Crossandra infundibuliformis (L.) showcases promising bioactivity, including antioxidant, antibacterial and wound healing properties, making it a valuable candidate for further exploration in pharmaceutical and healthcare applications. This study provides a comprehensive understanding of the medicinal potential of this plant and sets the stage for future research and development.