Solid lipid Nanoparticles (SLN) have emerged as a targeted drug delivery system with several applications within the field of pharmaceuticals, cosmetics, research, clinical drugs and different allied sciences. The review comprises an overview on the potential advantages and the disadvantages of solid lipid nanoparticles, drug incorporation models, method of preparation, secondary production steps, characterization, application and drug toxicity of solid lipid nanoparticles. SLN comprises the solid and lipid materials which were incorporated and by producing them as a Nanoparticle it is widely used in many of the disease conditions nowadays. Different production strategies that area unit appropriate for big scale production and applications of solid macromolecule nanoparticles area unit delineated. There is a specific analytical techniques for characterization of solid nanoparticles like Scanning electron microscopy (SEM) and differential scanning calorimetry a. If fittingly investigated, solid macromolecule nanoparticles might open new vistas in medical aid of advanced diseases.

A new, simple, precise, accurate and reproducible RP-HPLC and UV method for stability indicating method development and validation of Ledipasvir and Sofosbuvir in bulk form. Separation of Ledipasvir and Sofosbuvir was successfully achieve Dona Zorbax C8, 250 X4.6, 5µm or equivalent in an isocratic mode utilizing 0.1% OPA: Methanol (45:55) at a flow rate of 1.0mL/min and eluate was monitored at 238nm, with a retention time of 3.296 and 7.257minutes for Ledipasvir and Sofosbuvir respectively. The method was validated and their response was found to be linear in the drug concentration range of 45µg/ml to135µg/ml for Ledipasvir and 200µg/ml to 600µg/ml for and Sofosbuvir. The values of the correlation coefficient were found to 0.999 for Ledipasvir and 1 for Sofosbuvir respectively. The LOD and LOQ for Ledipasvir were found to be 0.090 and 0.695 respectively. The LOD and LOQ for Sofosbuvir were found to be 0.6619 and 2.2063 respectively. This method was found to be good percentage recovery for Ledipasvir and Sofosbuvir were found to be 100 and 100 respectively indicates that the proposed method is highly accurate. The specificity of the method shows good correlation between retention times of standard with the sample so, the method specifically determines the analyte in the sample without interference from excipients of tablet dosage forms. The method was extensively validated according to ICH guidelines for Linearity, Accuracy, Precession, Specificity and Robustness.

Mangifera indica commonly known as mango tree that are an important source of antioxidant molecules such as phenolic compounds and also it is used as traditional medicine for the treatment of skin irritations diseases. In this present study we carried out the antioxidant and wound healing activity of the extract by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Superoxide scavenging activity. Evaluation of antioxidant activity was performed at 125, 250, 500 and 1000µg/mL of EEMI concentrations. In DPPH assay EEMI showed the maximum antioxidant effect (68.04%) and Superoxide scavenging activity the extract has showed (74.92%) as compared to the standard (67.83, 97.01%). In vivo incision wound healing activity of EEMI ointment concentrations (2.5% (w/w) and 5% (w/w)) were tested in the rats. The wound contraction effect was measured every 2 days of 15 days of post wounding days. The standard drug silver sulfadiazine 10(w/w) showed a complete healing effect on 12th day of wound excision whereas, test extract EEMI 2.5% (w/w), 5% (w/w) showed healing effect on 15th and 14th day respectively. These findings are revealed that ethanol extract of M.indica showed variable degrees of antioxidant activity and significantly enhanced the percentage of wound contraction and the period of epithelialization comparable to standard silver sulfadiazine. These results demonstrate a positive effect of EEMI on the wound healing process on wound contraction is possibly due to the antioxidant activity exhibited by phytochemicals in the selected plant extract.

The nature has provided a complete depot of remedies to cure sicknesses of manhood. About 80 - 90 per cent of the world's population depends partly or exclusively on traditional medicine for its major health care needs. Herbal or plant medicines as the foremost therapy in traditional medical system have been used in clinical practice for many of years and have made a great support to maintain human health care. The present study revealed that a detailed pharmacognostical and phytochemical, morphological and microscopical and histological studies of the leaf stem and fruit, of the plant, Sesbania procumbens. The parameters presented in this study may be proposed to create the reality of leaf, root, stem and fruit of Sesbania procumbens and it may probably aid to differentiate the phytochemical, morphological and microscopical from its other species.

There are now more than 37 million confirmed cases and around 1.08 million deaths being reported of COVID-19 worldwide. The disease has reached 190 countries, with the USA has the extremely affected country in the world followed by India, Brazil. Pregnant women are referred to be endangered group owing to the immunologic and physiologic changes over expressed during pregnancy that can increase the risk of severe maternal disease could lead to incidence of preterm and caesarean births. However, the risk in pregnancy not well known more data should be needed. Vertical transmission might be possible in some theoretical model through ACE2 receptor widely expressed in the placenta. Few anti-viral drugs like lopinavir + ritonavir, remdesivir along with hydroxychloroquine has contained some efficacy but further proof needed through large number of clinical trials. Vaccines trail also should be specified in pregnant women because current studies excluded pregnant women.