Dunaliella salina are exploited for various purposes of which extraction of glycerol and β carotenes are the most important. Considering the sustainability, environmental friendly nature and steady release of nutrients, organic fertilizers could be used for the cultivation of these species. The organic fertilizer 6 DRIPTM was chosen for this purpose. Different concentrations of this medium (0.2-1 ml/L) were provided to grow these species and the growth rate was recorded for 16 days by measuring cell count and dry weight. The maximum dry weight (3.70 mg/L) cell counts (17726 cells/ml) and mean growth rate (0.063 divisions/day) obtained for 0.8 ml/L concentration of 6 DRIPTM were similar to those obtained from Dewalne’s medium (maximum dry weight 3.40 mg/L, maximum cell count 11280 cells/ml, mean growth rate 0.64 divisions/day). These samples analyzed in which the percentage of various nutrients present was recorded. β carotene level was found to be 21.89 g/100g (21.89%) of sample, which is around 21% of the dry weight. β carotene: Chlorophyll ratio was found to be 10:1 which is significant. Also, carbohydrate content was 20.18%; crude protein content was 12.20% and total lipid content was 30% of the dry biomass respectively. Thus, considering the growth rate and nutrient profile obtained for the Dunaliella salina grown in 6 DRIPTM, this can be considered as an effective and environment friendly growth medium for the same.
Inflammation is a localized reaction that produces redness, warmth, swelling, pain due to infection, irritation, and/or injury. Inflammation can be external or internal. Ferula Asafoetida Linn [Family: Umbelliferae] has been used, as anti -inflammatory agent in traditional system of medicine in India. However, there are no reports about its scientific validation for the claimed activity. Results: Preliminary photochemical investigation of the hydroalcoholic extract of Ferula Asafoetida Linnrootreveals the presence of carbohydrates, proteins, alkaloids, flavonoids, phytosterols, tannins and volatile oils. In group of carrageen an induced paw edema in rats, there is highly significant decreased paw volume when treated with Diclofenac (1 ± 0.**) and high dose of Ferula Asafoetidaroot extract (1.666 ± 0.333**) in the comparison with controlled animals (3.333±0.333). This comparison was found to be highly significant (p<0.01). In this method, there was significant decreased paw volume when treated with low dose of Ferula Asafoetida Linnroot extract (2±0) in the comparison with control animals (3.333±0.333). This comparison was found to be significant (p<0.05). Conclusion: The present investigation revealed that, the Ferula Asafoetida Linnroot extract at high and low doses produces highly significant and significant decreased in carrageenan induced paw edema in rats. This activity may be due to presence of flavonoids, phytosterols and tannins in extract.
Venkateswarlu Guddeti*, J. N. Suresh Kumar, D. Naga Rani, I. Purnananda Reddy, K. V. L. Likhitha, K. Sandeep.
It’s a fact that elevated TG’s, LDL and decreased HDL in serum are major risk factor for coronary artery disease, it became necessary to establish the effect of oil consumption on lipid profile. In spite of all business making publicity stunts by oil manufactures choosing best oils from wide variety of availability is very difficult for public so, we try to establish cardiac friendliness of oils by conducting animal studies on albino rats. 30 albino male rats were used in this study. The animals are randomly allocated into 5 groups (each group of 5) of approximately equal average body weight (150-200gm). In this study rat feed is prepared by mixing 15 ml of oil for every 85 gm. of feed and rats are feeded for duration of 28 days. At the end of the experimental period (7-8 weeks) animals were fasted for 12hrs and blood samples are collected Lipid profile is conducted and results are compared with std reference values. From this study we concluded that the TC levels are elevated on use of Groundnut oil comparing to other oils, HDL levels are elevated on use of olive oil comparing to other oils, The LDL levels are elevated on use of groundnut oil and low in fish oil, The TG levels are decreased than normal in case of all oil but fish oil shows TG level near to normal group. Finally we conclude that fish oil is cardiac friendly showing good impact on lipid profile as it shows low TC, LDL levels and high HDL level comparing to control. Next to that olive oil is showing better impact on lipid profile.
K. Mallikarjuna Reddy*, G. Venkateswarlu, G. Swaruparani, J. N. Suresh Kumar.