Amoxicillin, an acid stable, semi‐synthetic drug belongs to a class of antibiotics called the Penicillins (beta‐lactam antibiotics). It is shown to be effective against a wide range of infections caused by wide range of Gram ‐positive and Gram‐ negative bacteria in both human and animals. Tinidazole is a prodrug and antiprotozoal agent. Both the drugs are used to treat Gastro intestinal infectious diseases and upper respiratory tract infections. Techniques like UV-Visible spectrophotometry, potentiometry, High Performance Liquid Chromatography (HPLC), High performance Thin Layer Chromatography (HPTLC) etc have been used for analysis. UV-Visible spectrophotometry and HPLC methods have been used most widely.

Reproduction is initiated by mating of male with a female in sexual intercourse which facilitates the coming together of sperm and egg for the purpose of fertilization. Sexual dysfunction effectively is a major problem facing the reproductive process. In ayurvedic medicine Punica granatum (Linn) is a fruit-used as aphrodisiac. Punica granatum (Linn) extract showed significant equal effect in sperm count and % of sperm motility when compared to Sildenafil and Testosterone administered rats, significant increase in sperm count and % of sperm motility when compared to Stressed rats. In case of histopathology of testis, treatment of Punica granatum (Linn.) extract in the rats overcomes the single layer of spermatogenesis, congested blood vessels and absence of spermatozoa due to stress and produces Thickening of basement membrane, predominance of spermatocytes, increase in no.of sertoli cells and maturation arrest in few seminiferous tubule cells.

The root Daucuscarota belongs to family Araceae which is known as Carrot in Telugu. In the present study isolated piperine from Daucuscarota fruit were subjected to the phytochemical investigation and evaluated for antidiabetic activity on blood glucose level, lipid profiles and on the body weight in streptozotocin induced diabetic rats. Isolated piperine (100mg/kg) from Daucuscarota extract and Glibenclamide (10mg/kg) were administered orally in streptozotocin (50mg/kg, i.p.) induced diabetic rats. In this antidiabetic study, maximum reduction in blood glucose was observed in isolated piperine (160.8, 96.7mg/dl) at the dose of 100mg/kg on 21stday respectively. The isolated piperine showed the significant effect (p<0.005) in the various biochemical parameters like protein, triglycerides, cholesterol and total lipid levels. Isolated piperine (100mg/kg) was found to have significant (p<0.001) blood glucose lowering effect. Preliminary Phytochemical investigation revealed the presence of alkaloids, as the major constituents in the Daucuscarota plant. These results suggest that piperine (100mg/kg) showed antidiabetic activity in streptozotocin induced diabetic rats.